Lower risk of graft dislocation applying organ cultured corneas compared with cold short-term cultured corneas during DSAEK surgery
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چکیده
منابع مشابه
Use of poloxamers for deswelling of organ-cultured corneas.
PURPOSE Dextran T500, routinely used as a deswelling supplement in organ culture (OC), has been suspected of being toxic to corneal endothelial cells (ECs). This study was conducted to evaluate the innovative use of poloxamers compared with dextran for deswelling OC corneas. METHODS Five poloxamers (P124, P188, P237, P338, and P407) were dissolved respectively in a standard OC medium to reach...
متن کاملFactors influencing the suitability of organ-cultured corneas for transplantation.
PURPOSE To assess the influence of donor and storage factors on the suitability of organ-cultured corneas for penetrating keratoplasty (PKP) using multifactorial regression analysis. METHODS Corneas (mean donor age, 57 years; standard deviation, 21 years) were stored by organ culture at 34 degrees C for up to 5 weeks (mean, 22 days; standard deviation, 6 days). The endothelium was assessed by...
متن کامل4215 Matrix-metalloprotease activation in cultured corneas
Purpose. Vascular endothelial growth factor (VEGF) binds to oorneal endothelial cells (BCE). We screened BCE for the expression of the VEGF receptors KDRIFLK-I and FLT-1 and analysed their functions. Methods. The expression of KDRIFLK-1 and FLT-1 mRNA was analysed by northern-blot. BCE were also transfeoted with expression vectors carriing the VEGF receptors sequences. Their ability to prolifer...
متن کاملVery early endothelial cell loss after penetrating keratoplasty with organ-cultured corneas.
AIMS After keratoplasty, postoperative endothelial cell loss is calculated between the eye bank endothelial cell density (ebECD) and the postoperative specular microscopy (SM). To elucidate the very early cell loss, always described after penetrating keratoplasty (PK), we designed two complementary studies. METHODS (1) Clinical prospective study of 90 consecutive PKs (keratoconus, Fuchs' corn...
متن کاملEndothelial Morphometry by Image Analysis of Corneas Organ Cultured at 31°C
METHODS. The endothelia of 505 of the 559 corneas consecutively stored at the eye bank were routinely analyzed with Sambacornea image-analysis software (ver. 1.2.10; Tribvn, Châtillon, France) on three large-field images of 75
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ژورنال
عنوان ژورنال: Eye
سال: 2019
ISSN: 0950-222X,1476-5454
DOI: 10.1038/s41433-019-0579-8